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To construct the plasmid encoding the luciferase gene driven by XBP1 binding sites, a synthetic oligonucleotide containing four XBP1 binding sites, 5К№ aagctagccgcgTGGA GCCACGT TACATGGA GCCACGT TACATGGA GCCACGT TACATGGA GCCACGT TACAaagctttt 3К№ italicized sequences represent the XBP1 binding sites, was digested with NheI HindIII and the ligated into the pGL3 vector generic cialis